Hyphenated SeparationTechniques

Hybrid separation techniques or hyphenated techniques is that the combination of mass spectrometry and chromatography within which the particles may be detected then separated consequently by chromatographic separation technique. Typically, this can be often helpful for pre-isolation analyses of crude extracts or fraction from varied natural sources, isolation and on-line detection of a natural product, chemotaxonomic studies, chemical fingerprinting, quality control of the herbal product, depreciation of natural product, and metabolomics.


  • Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)
  • Gas chromatography-infrared spectroscopy (GC-IR)
  • HPLC-ESI-MS / HPLC-CE-MS - mass spectrometry
  • Liquid chromatography-NMR spectroscopy (LC-NMR)
  • LC-NMR-MS
  • Liquid chromatography-infrared spectroscopy(LC-IR)
  • Applications of hyphenated separation techniques

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